New localities and current distribution maps of new, rare and infrequent vascular plants in the Puszcza Niepołomicka forest and adjacent areas (between the Vistula and Raba rivers) are presented. Chenopodium hybridum, Falcaria vulgaris, Myosotis discolor, Rosa gallica, Thlaspi perfoliatum, Veronica polita and Viola odorata are first records for this area. Further sites of very rare and rare species were found, including Barbarea stricta, Bromus erectus, Cerastium glutinosum, C. semidecandrum, Equisetum pratense, Fragaria viridis, Holosteum umbellatum, Lithospermum arvense, Myosotis ramosissima, M. stricta, Potentilla neumanniana, Trifolium arvense, Valerianella locusta, Viola hirta and V. stagnina.